VIPSolutions890 ul + 0.001 L -0.8 ml -1000 ul. = (in ul) 335 ul + 0.00015 L - 0.60 ml +0.001 L = (in mi) 7.5 ml +500 ul -0.003L + 0.0025L= (in L) Section Ill. Complete the following table, make the standard curve ( correctly labeled) and calculate the concentration of the unknown sample. SHOW ALL CALCULATIONS! Solute(ml) Solvent(ml) | Dilution Concentration Absorbance (ug/ml) 16 0 200 0514 14 2 40 0235 12 4 20 073 10 6 10 0171 8 8 5 015 6 10 25 0153 4 12 1 0.149 2 14 05 0.148 0 16 0 0.145 Unknown sample absorbance= 0.281
Question:
890 ul + 0.001 L -0.8 ml -1000 ul. = (in ul)
335 ul + 0.00015 L - 0.60 ml +0.001 L = (in mi)
7.5 ml +500 ul -0.003L + 0.0025L= (in L)
Section Ill. Complete the following table, make the standard curve ( correctly labeled) and calculate the concentration of the
unknown sample. SHOW ALL CALCULATIONS!
Solute(ml) Solvent(ml) | Dilution Concentration Absorbance
(ug/ml)
16 0 200 0514
14 2 40 0235
12 4 20 073
10 6 10 0171
8 8 5 015
6 10 25 0153
4 12 1 0.149
2 14 05 0.148
0 16 0 0.145
Unknown sample absorbance= 0.281
This is AI generated Response.
Asked by: Nikita Gaikwad
Created at: 2025-06-17 19:47:06
Asked by: Nikita Gaikwad
Created at: 2025-06-17 19:47:06
Final Answer:
Rendered
Raw Text
Did you like this answer?
Let's go step by step!
### **Step 1: Complete the Table**
#### **A. Dilution Calculation**
Dilution factor = Total volume / Solute volume
Total volume = Solute (ml) + Solvent (ml)
#### **B. Table Completion**
Let's fill in the dilution column.
| Solute (ml) | Solvent (ml) | Total Volume (ml) | Dilution Factor (Total/Solute) | Concentration (µg/ml) | Absorbance |
|-------------|--------------|-------------------|-----------------------------|----------------------|------------|
| 16 | 0 | 16 | 16/16 = 1 | 200 | 0.514 |
| 14 | 2 | 16 | 16/14 ≈ 1.14 | 40 | 0.235 |
| 12 | 4 | 16 | 16/12 ≈ 1.33 | 20 | 0.173 |
| 10 | 6 | 16 | 16/10 = 1.6 | 10 | 0.171 |
| 8 | 8 | 16 | 16/8 = 2 | 5 | 0.150 |
| 6 | 10 | 16 | 16/6 ≈ 2.67 | 2.5 | 0.153 |
| 4 | 12 | 16 | 16/4 = 4 | 1 | 0.149 |
| 2 | 14 | 16 | 16/2 = 8 | 0.5 | 0.148 |
| 0 | 16 | 16 | 16/0 = ∞ | 0 | 0.145 |
---
### **Step 2: Standard Curve**
- Plot **Concentration (x-axis)** vs **Absorbance (y-axis)**.
- Fit a straight line (since Beer's Law: A = εlc, absorbance is proportional to concentration).
#### **Linear Regression Equation**
You can use Excel or a calculator for best-fit line, but let's estimate using two points:
Let's use points (200, 0.514) and (0, 0.145):
Slope (m) = (0.514 - 0.145) / (200 - 0) = 0.369 / 200 ≈ 0.001845
Intercept (b) = 0.145 (when concentration is zero)
Equation:
**Absorbance = 0.001845 × Concentration + 0.145**
---
### **Step 3: Find Unknown Concentration**
Given:
Unknown Absorbance = 0.281
Use the equation:
0.281 = 0.001845 × C + 0.145
Solve for C:
0.281 - 0.145 = 0.001845 × C
0.136 = 0.001845 × C
C = 0.136 / 0.001845 ≈ **73.7 µg/ml**
---
## **Summary Table (with Dilution Added)**
| Solute (ml) | Solvent (ml) | Dilution Factor | Concentration (µg/ml) | Absorbance |
|-------------|--------------|-----------------|------------------------|------------|
| 16 | 0 | 1 | 200 | 0.514 |
| 14 | 2 | 1.14 | 40 | 0.235 |
| 12 | 4 | 1.33 | 20 | 0.173 |
| 10 | 6 | 1.6 | 10 | 0.171 |
| 8 | 8 | 2 | 5 | 0.150 |
| 6 | 10 | 2.67 | 2.5 | 0.153 |
| 4 | 12 | 4 | 1 | 0.149 |
| 2 | 14 | 8 | 0.5 | 0.148 |
| 0 | 16 | ∞ | 0 | 0.145 |
---
## **Unknown Sample Calculation**
**Absorbance = 0.281**
**Equation: Absorbance = 0.001845 × Concentration + 0.145**
**Concentration ≈ 73.7 µg/ml**
---
If you need the standard curve plotted, let me know!
📢 Instructions:
- Ask anything relatd to above question only.
- For new Question goto :