(3) Agitator Power Four baffles and a two-layer, six-curved-blade turbine agitator are installed to ensure efficient mixing. To select and procure a suitable drive motor, calculate agitation power (P)and the agitation power (Pg) with aeration using Michel's formula for this pilot-scale tank.Problem-Solving Question: A recombinant lipase enzyme is produced using a recombinant, aerobic filamentous fungus. necessitating an oxygen-rich environment during fermentation. For the pilot-scale process, an aerated stirred-tank reactor with a height-to-diameter ratio (H/D) of 2:1 is employed. The tank is equipped with a two-layer. six-curved-blade turbine agitator, where each impeller has a diameter of 0.5 m. To accommodate headspace and foam management, the bioreactor is operated at 64% of its nominal volume. The parameters of a pilot tank are as follows: Pilot Parameter Unit “Tank diameter) 1 om Tank height (H) 2 m Loading factor 64 % Impeller diameter (d) 0.5 m Agitation speed 300 pm Fermentation time 180 h 1 al nema) « b Gauge pressure of tank 03 MPa Temperature in tank 28 °C Aeration rate 12 VVM (standard conditions) Broth viscosity 0.1 Pas Broth density 1050 kg/m? Final lipase concentration in broth ~~ 18.18 gL
Question:
(3) Agitator Power Four baffles and a two-layer, six-curved-blade turbine agitator are installed to ensure efficient mixing. To select and procure a suitable drive motor, calculate agitation power (P)and the agitation power (Pg) with aeration using Michel's formula for this pilot-scale tank.
Problem-Solving Question: A recombinant lipase enzyme is produced using a recombinant,
aerobic filamentous fungus. necessitating an oxygen-rich environment during fermentation. For the
pilot-scale process, an aerated stirred-tank reactor with a height-to-diameter ratio (H/D) of 2:1 is
employed. The tank is equipped with a two-layer. six-curved-blade turbine agitator, where each
impeller has a diameter of 0.5 m. To accommodate headspace and foam management, the bioreactor
is operated at 64% of its nominal volume.
The parameters of a pilot tank are as follows:
Pilot Parameter Unit
“Tank diameter) 1 om
Tank height (H) 2 m
Loading factor 64 %
Impeller diameter (d) 0.5 m
Agitation speed 300 pm
Fermentation time 180 h
1 al
nema) « b
Gauge pressure of tank 03 MPa
Temperature in tank 28 °C
Aeration rate 12 VVM (standard conditions)
Broth viscosity 0.1 Pas
Broth density 1050 kg/m?
Final lipase concentration in broth ~~ 18.18 gL
Asked by: vemuri swathi
Created at: 2025-12-18 01:57:59
Final Answer:
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